Epithelium-dependent effect of L-glutamate on airways: involvement of prostaglandins.

نویسندگان

  • Apostolia A Hatziefthimiou
  • Konstantinos I Gourgoulianis
  • Paschalis-Adam Molyvdas
چکیده

We investigated the effect of the excitatory amino acid (EAA) receptor agonists L-glutamate, N-methyl-D-aspartate (NMDA), (RS)-a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and kainic acid on KCl-induced contractions of rabbit tracheal smooth muscle, as well as the role of epithelium and endogenously produced nitric oxide and prostaglandins on these responses. L-Glutamate decreased KCI-induced contractions up to 30%. This effect was attenuated by epithelium removal, tetrodotoxin, methylene blue and indomethacin but not by NG-nitro-L-arginine methyl ester. While NMDA, AMPA and kainic acid had no effect, the combination of NMDA + kainic acid decreased KCI-induced contractions. These results suggest that, in rabbit trachea, L-glutamate has, at least in part, an epithelium-dependent effect mediated via prostaglandin formation and that the EAA receptors involved are non-classical.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Asymmetric Involvement of Central and the Peripheral NMDA Glutamate Receptors in the Expression of Withdrawal Syndrome in Morphine-Dependent Mice

Introduction: Morphine withdrawal syndrome is mediated via several central and peripheral neurological pathways. In the present study we investigated the role of N-methyl-D aspartic acid (NMDA) glutamate receptor on naloxone-induced withdrawal syndrome in morphine-conditioned mice. Materials and Methods: We designed two separate experiments. In experiment one, 30 male NMRI mice were divide...

متن کامل

Epithelium-dependent regulation of airways smooth muscle function. A histamine-nitric oxide pathway.

The airway epithelium is responsible for the production of a number of arachidonic acid and non-prostanoid inhibitory factors. Epithelium synthesises nitric oxide (NO) which may be important in regulating the function of airways smooth muscles. We studied in vitro the effect of histamine (100 nM-100 microM) which increases the NO release on rabbit airway smooth muscles induced by 80 mM KC1 in t...

متن کامل

Bronchodilatory Effect of Hydrogen Sulfide in Rat

Objective(s) The aims of present study were to elucidate the effect of NaHS as a H2S donor on precontracted rat trachea smooth muscle, role of epithelium and nitric oxide in this action. Materials and Methods Tracheal rings from male adult Wistar rats were isolated and mounted in an organ bath containing Krebs–Henseleit solution under 1.5 g resting tension and contractions were recorded isom...

متن کامل

Involvement of nitric oxide within the rat central nucleus of amygdala in morphine tolerance

The role of glutamate receptor within the nucleus accumbens in morphine tolerance has been postulated. Previous studies have reported that glutamate receptors exert their effects in part through the release of nitric oxide (NO). In the present study the effects of intra-accumbal injections of L-arginine (0.3, 1, and 3 ?g/rat), the NO precursor and L-NAME (0.3, 1, and 3 ?g/rat), the NOS inhibito...

متن کامل

Involvement of nitric oxide within the rat central nucleus of amygdala in morphine tolerance

The role of glutamate receptor within the nucleus accumbens in morphine tolerance has been postulated. Previous studies have reported that glutamate receptors exert their effects in part through the release of nitric oxide (NO). In the present study the effects of intra-accumbal injections of L-arginine (0.3, 1, and 3 ?g/rat), the NO precursor and L-NAME (0.3, 1, and 3 ?g/rat), the NOS inhibito...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Mediators of Inflammation

دوره 11  شماره 

صفحات  -

تاریخ انتشار 2002